Glycosylation of acetylxylan esterase from Trichoderma reesei
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چکیده
منابع مشابه
Ethanol Perturbs Glycosylation and Inhibits Hypersecretion in Trichoderma reesei.
The effects of ethanol and phenylethanol on the growth of and glycoprotein secretion by Trichoderma reesei were studied. Low levels (1.5%, vol/vol) of ethanol perturbed the glycosylation process, as shown by alterations in the isoelectric profile of the secreted proteins and a reduction in the rate of incorporation of mannose into oligosaccharides. In addition to these effects on posttranslatio...
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A systematic analysis of the N-glycosylation of the catalytic domain of cellobiohydrolase I (Cel7A or CBH I) isolated from several Trichoderma reesei strains grown in minimal media was performed. Using a combination of chromatographic, electrophoretic, and mass spectrometric methods, the presence of glucosylated and phosphorylated oligosaccharides on the three N-glycosylation sites of Cel7A cor...
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Endoglucanase III (EG III) was purified to homogeneity from the culture medium of Trichoderma reesei QM 9414. It has a molecular mass of 48 kDa, and an isoelectric point of 5.1. Maximal activity was observed between pH4 and 5. Celloligosaccharides and their chromophoric derivatives were used as substrates, and the reaction products were analysed by quantitative h.p.l.c. Nucleophilic competition...
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Hydrophobins are a group of small amphiphilic proteins which are known to self-assemble on interfaces. They contain eight conserved cysteine residues, which make four disulfide bridges. A new hydrophobin protein, HFBIII, from the fungus Trichoderma reesei contains one extra cysteine residue, giving the protein a naturally reactive site. The self-assembly of hydrophobin protein HFBIII was studie...
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The mesophilic filamentous fungus Trichoderma reesei (teleomorph Hypocrea jecorina) has become a major cell factory in the enzyme industry, and the benchmark organism for production of cellulases, especially for biomass conversion. The strain was originally isolated during the second World War from US Army tent canvas in the Solomon Islands and initially identified as Trichoderma viride. The is...
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ژورنال
عنوان ژورنال: Glycobiology
سال: 2002
ISSN: 0959-6658,1460-2423
DOI: 10.1093/glycob/12.4.291